Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 32 publications below |
| IHC | See 2 publications below |
Product Information
17548-1-AP targets HDAC8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11696 Product name: Recombinant human HDAC8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC050433 Sequence: MEEPEEPADSGQSLVPVYIYSPEYVSMCDSPAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV 相同性解析による交差性が予測される生物種 |
| Full Name | histone deacetylase 8 |
| Calculated molecular weight | 377 aa, 42 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC050433 |
| Gene Symbol | HDAC8 |
| Gene ID (NCBI) | 55869 |
| RRID | AB_2116926 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BY41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HDAC8 antibody 17548-1-AP | Download protocol |
| IHC protocol for HDAC8 antibody 17548-1-AP | Download protocol |
| IP protocol for HDAC8 antibody 17548-1-AP | Download protocol |
| WB protocol for HDAC8 antibody 17548-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors. | ||
Cell Rep Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex. | ||
Kidney Int Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. | ||
Oncotarget Thrombospondin-1 might be a therapeutic target to suppress RB cells by regulating the DNA double-strand breaks repair. | ||
Oncogenesis USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3. | ||
Molecules Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway |
