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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HCN1 Recombinant antibody (84845-3-RR)

    WB analysis using 84845-3-RR

    Boiled and unboiled mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 84845-3-RR (HCN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HCN1 Recombinant antibody (84845-3-RR)

    IHC staining of mouse cerebellum using 84845-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84845-3-RR (HCN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HCN1 Recombinant antibody

HCN1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84845-3-RR
Clone No.242254A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, HAC 2, Brain cyclic nucleotide-gated channel 1, BCNG-1, 242254A1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84845-3-RR targets HCN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name hyperpolarization activated cyclic nucleotide-gated potassium channel 1
Calculated molecular weight 99 kDa
Observed molecular weight 99-120 kDa
GenBank accession numberNM_021072
Gene Symbol HCN1
Gene ID (NCBI) 348980
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60741
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HCN1, also named as BCNG1, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. HCN1 contributes to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. HCN1 may mediate responses to sour stimuli.

Protocols

Product Specific Protocols
WB protocol for HCN1 antibody 84845-3-RRDownload protocol
IHC protocol for HCN1 antibody 84845-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols