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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HCN1 Recombinant antibody (84845-3-RR)

    WB analysis using 84845-3-RR (same clone as 84845-3-PBS)

    Boiled and unboiled mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 84845-3-RR (HCN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84845-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HCN1 Recombinant antibody (84845-3-RR)

    IHC staining of mouse cerebellum using 84845-3-RR (same clone as 84845-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84845-3-RR (HCN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84845-3-PBS in a different storage buffer formulation.

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HCN1 Recombinant antibody, PBS Only

HCN1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84845-3-PBS
Clone No.242254A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, HAC 2, Brain cyclic nucleotide-gated channel 1, BCNG-1, 242254A1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84845-3-PBS targets HCN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name hyperpolarization activated cyclic nucleotide-gated potassium channel 1
Calculated molecular weight 99 kDa
Observed molecular weight 99-120 kDa
GenBank accession numberNM_021072
Gene Symbol HCN1
Gene ID (NCBI) 348980
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60741
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HCN1, also named as BCNG1, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. HCN1 contributes to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. HCN1 may mediate responses to sour stimuli.