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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HBE1-Specific Monoclonal antibody

HBE1-Specific Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C11G6

Cat no : CL488-66151

Synonyms

Epsilon globin, HBE, HBE1, HBE1-Specific, Hemoglobin epsilon chain, Hemoglobin subunit epsilon, hemoglobin, epsilon 1

Validation Data Gallery

Applications:
  • FC experiment of K-562 using CL488-66151

    FC experiment of K-562 using CL488-66151

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HBE1-Specific (CL488-66151, Clone:2C11G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of SH-SY5Y using CL488-66151

    FC experiment of SH-SY5Y using CL488-66151

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HBE1-Specific (CL488-66151, Clone:2C11G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of THP-1 using CL488-66151

    FC experiment of THP-1 using CL488-66151

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HBE1-Specific (CL488-66151, Clone:2C11G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inK-562 cells, SH-SY5Y cells, THP-1 cells
For other applications, we recommend the unconjugated version of this antibody, 66151-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66151 is HBE1-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name hemoglobin, epsilon 1
Observed molecular weight 16 kDa
GenBank accession numberNM_005330
Gene symbol HBE1
Gene ID (NCBI) 3046
RRIDAB_2919291
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 HBE1-Specific antibody CL488-66151Download protocol
Standard Protocols
Click here to view our Standard Protocols