Validation Data Gallery
Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293T cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 43 publications below |
| IF | See 6 publications below |
| IP | See 16 publications below |
| CoIP | See 7 publications below |
Product Information
81290-1-RR targets HA Tag in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | HA Tag |
| Calculated molecular weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2935602 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | HATAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for HA Tag antibody 81290-1-RR | Download protocol |
| WB protocol for HA Tag antibody 81290-1-RR | Download protocol |
| FC protocol for HA Tag antibody 81290-1-RR | Download protocol |
| IF protocol for HA Tag antibody 81290-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Angew Chem Int Ed Engl Small molecule-inducible and photoactivatable cellular RNA N1-methyladenosine editing | ||
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Cell Rep Med Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity | ||
Adv Sci (Weinh) PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer | ||
Theranostics LINC00982-encoded protein PRDM16-DT regulates CHEK2 splicing to suppress colorectal cancer metastasis and chemoresistance |
