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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HA Tag Recombinant monoclonal antibody (81290-1-RR)

    WB analysis using 81290-1-RR (same clone as 81290-1-PBS)

    HA-tag recombinant protein, non-transfected HEK-293 cells, transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81290-1-RR (HA Tag antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293T cells using HA Tag Recombinant monoclonal antibody (81290-1-RR)

    IP experiment of Transfected HEK-293T using 81290-1-RR (same clone as 81290-1-PBS)

    IP result of anti-HA Tag (IP:81290-1-RR, 4ug; Detection:81290-1-RR 1:10000) with Transfected HEK-293T cells lysate 400 ug. This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA Tag Recombinant monoclonal antibody (81290-1-RR)

    IF Staining of Transfected HEK-293 using 81290-1-RR (same clone as 81290-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (81290-1-RR, Clone: 6I21 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA Tag Recombinant monoclonal antibody (81290-1-RR)

    IF Staining of Transfected HEK-293 using 81290-1-RR (same clone as 81290-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using HA Tag antibody (81290-1-RR, Clone: 6I21 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Transfected HEK-293 cells using HA Tag Recombinant monoclonal antibody (81290-1-RR)

    FC experiment of Transfected HEK-293 using 81290-1-RR (same clone as 81290-1-PBS)

    1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug HA Tag Recombinant antibody (81290-1-RR, Clone:6I21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81290-1-RR

    Affinity and Kinetic Characterization of 81290-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81290-1-RR against a protein with HA tag were performed. The affinity constant is 0.766 nM.

HA Tag Recombinant monoclonal antibody, PBS Only

HA Tag Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 81290-1-PBS
Clone No.6I21

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

HA

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81290-1-PBS targets HA Tag in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name HA Tag
Calculated molecular weight 1 kDa
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDHATAG
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Protocols

Product Specific Protocols
IP protocol for HA Tag antibody 81290-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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