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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Granzyme K Monoclonal antibody

Granzyme K Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3A4

Cat no : CL488-67272

Synonyms

Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL488-67272

    IF Staining of human tonsillitis using CL488-67272

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 Granzyme K antibody (CL488-67272, Clone: 1C3A4 ) at dilution of 1:200. DAPI (blue).

  • FC experiment of NK92 using CL488-67272

    FC experiment of NK92 using CL488-67272

    1X10^6 NK92 were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Granzyme K (CL488-67272, Clone:1C3A4) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inNK92, NK92 cells
For other applications, we recommend the unconjugated version of this antibody, 67272-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67272 targets Granzyme K in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Granzyme K fusion protein Ag27297 相同性解析による交差性が予測される生物種
Full Name granzyme K (granzyme 3; NK tryptase II)
Calculated molecular weight 29 kDa
GenBank accession numberBC035802
Gene symbol GZMK
Gene ID (NCBI) 3003
RRIDAB_2919442
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Granzyme K antibody CL488-67272Download protocol
FC protocol for CL Plus 488 Granzyme K antibody CL488-67272Download protocol
Standard Protocols
Click here to view our Standard Protocols