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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GTSE1 Polyclonal antibody (21319-1-AP)

    WB analysis using 21319-1-AP (same clone as 21319-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GTSE1 Polyclonal antibody (21319-1-AP)

    WB analysis using 21319-1-AP (same clone as 21319-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GTSE1 Polyclonal antibody (21319-1-AP)

    WB analysis using 21319-1-AP (same clone as 21319-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GTSE1 Polyclonal antibody (21319-1-AP)

    WB analysis of HeLa using 21319-1-AP (same clone as 21319-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GTSE1 Polyclonal antibody (21319-1-AP)

    IHC staining of human liver cancer using 21319-1-AP (same clone as 21319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GTSE1 Polyclonal antibody (21319-1-AP)

    IHC staining of human liver cancer using 21319-1-AP (same clone as 21319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using GTSE1 Polyclonal antibody (21319-1-AP)

    IHC staining of mouse spleen using 21319-1-AP (same clone as 21319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GTSE1 Polyclonal antibody, PBS Only

GTSE1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21319-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21319-1-PBS targets GTSE1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13497

Product name: Recombinant human GTSE1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 416-720 aa of BC006325

Sequence: CAWSESSQLNKTRSIRRRDSCLNSKTKVMPTPTNQFKIPKFSIGDSPDSSTPKLSRAQRPQSCTSVGRVTVHSTPVRRSSGPAPQSLLSARRVSALPTPASRRCSGLPPMTPKTMPRAVGSPLCVPARRRSSEPRKNSAMRTEPTRESNRKTDSRLVDVSPDRGSPPSRVPQALNFSPEESDSTFSKSTATEVAREEAKPGGDAAPSEALLVDIKLEPLAVTPDAASQPLIDLPLIDFCDTPEAHVAVGSESRPLIDLMTNTPDMNKNVAKPSPVVGQLIDLSSPLIQLSPEADKENVDSPLLKF

相同性解析による交差性が予測される生物種
Full Name G-2 and S-phase expressed 1
Calculated molecular weight 77 kDa
Observed molecular weight 66-77 kDa, 100 kDa
GenBank accession numberBC006325
Gene Symbol GTSE1
Gene ID (NCBI) 51512
RRIDAB_10733644
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYZ3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.

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