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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated GSTP1 Polyclonal antibody

GSTP1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-15902

Synonyms

DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI

Validation Data Gallery

Applications:
  • FC experiment of HepG2 using CL647-15902

    FC experiment of HepG2 using CL647-15902

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human GSTP1 (CL647-15902' target='_blank'>CL647-15902) (red), or 0.2 ug Rabbit IgG CL647 (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GSTP1 Ready-To-Use IHC Kit. GSTP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15902-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-15902 targets GSTP1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTP1 fusion protein Ag8731 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase pi 1
Calculated molecular weight 210 aa, 23 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC010915
Gene symbol GSTP1
Gene ID (NCBI) 2950
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutathione S-transferase P (GSTP1) conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 GSTP1 antibody CL647-15902Download protocol
Standard Protocols
Click here to view our Standard Protocols