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Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 647-conjugated GST Tag Polyclonal a (CL647-10000)

    FC experiment of Jurkat using CL647-10000

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-GST Tag (CL647-10000) (red), or 0.4 ug CoraLite® Plus 488-conjugated IGF2R antibody (CL488-20253) (green), or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 647-conjugated GST Tag Polyclonal antibody

GST Tag Polyclonal Antibody for FC (Intra)
Cat No. CL647-10000
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

FC (Intra)

Synonyms

Rabbit IgG control, GST, Glutathione S-Transferase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-10000 targets GST Tag in FC (Intra) applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883648
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen Affinity Purified
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 GST Tag antibody CL647-10000Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Viruses

Tumor Necrosis Factor and Interleukin-1β Upregulate NRP2 Expression and Promote SARS-CoV-2 Proliferation

Authors - Michinori Ishitoku
View Article