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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRK2 Polyclonal antibody (13990-1-AP)

    WB analysis using 13990-1-AP (same clone as 13990-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13990-1-AP (GRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using GRK2 Polyclonal antibody (13990-1-AP)

    IP experiment of HL-60 using 13990-1-AP (same clone as 13990-1-PBS)

    IP result of anti-GRK2 (IP:13990-1-AP, 4ug; Detection:13990-1-AP 1:1000) with HL-60 cells lysate 3040ug. This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using GRK2 Polyclonal antibody (13990-1-AP)

    IHC staining of human lymphoma using 13990-1-AP (same clone as 13990-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRK2 Polyclonal antibody (13990-1-AP)

    IHC staining of mouse brain using 13990-1-AP (same clone as 13990-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using GRK2 Polyclonal antibody (13990-1-AP)

    IHC staining of human lymphoma using 13990-1-AP (same clone as 13990-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GRK2 Polyclonal antibody (13990-1-AP)

    FC experiment of HeLa using 13990-1-AP (same clone as 13990-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug GRK2 Polyclonal antibody (13990-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13990-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GRK2 Polyclonal antibody, PBS Only

GRK2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 13990-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

ARBK1, ADRBK1, BETA ARK1, Beta ARK 1, BARK1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13990-1-PBS targets GRK2 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5071

Product name: Recombinant human ADRBK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 390-689 aa of BC037963

Sequence: PFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

相同性解析による交差性が予測される生物種
Full Name adrenergic, beta, receptor kinase 1
Calculated molecular weight 689 aa, 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC037963
Gene Symbol ADRBK1
Gene ID (NCBI) 156
RRIDAB_2225833
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP25098
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GRK2 (G-protein coupled receptor kinase 2) is also named as ADRBK1 (Beta-adrenergic receptor kinase 1), BARK, and BARK1. G-protein-coupled receptors (GPCRs) transmit extracellular signals across the cell membrane. GPCR kinases (GRKs) desensitize GPCR signals in the cell membrane. GRK2 translocation from the cytoplasm to the cell membrane in response to extracellular signals is critical to the function of the protein, where GRK phosphorylation is an important component of its translocation to the membrane.(PMID:27412951)

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