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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRK1 Recombinant antibody (85942-1-RR)

    WB analysis using 85942-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85942-1-RR (GRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse retina tissue using GRK1 Recombinant antibody (85942-1-RR)

    WB analysis of mouse retina using 85942-1-RR

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 85942-1-RR (GRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using GRK1 Recombinant antibody (85942-1-RR)

    IHC staining of mouse eye using 85942-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85942-1-RR (GRK1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using GRK1 Recombinant antibody (85942-1-RR)

    IHC staining of mouse eye using 85942-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85942-1-RR (GRK1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using GRK1 Recombinant antibody (85942-1-RR)

    IF Staining of mouse eye using 85942-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using GRK1 antibody (85942-1-RR, Clone: 250433A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85942-1-RR

    Affinity and Kinetic Characterization of 85942-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85942-1-RR against Human GRK1 were performed. The affinity constant is 3.77 nM.

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GRK1 Recombinant antibody

GRK1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85942-1-RR
Clone No.250433A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

EC:2.7.11.14, G protein-coupled receptor kinase 1, GPRK1, Rhodopsin kinase, Rhodopsin kinase GRK1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse eye tissue, mouse retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85942-1-RR targets GRK1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRK1 fusion protein Ag18063 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor kinase 1
Calculated molecular weight 563 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC140945
Gene Symbol GRK1
Gene ID (NCBI) 6011
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15835
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1, and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in the phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.

Protocols

Product Specific Protocols
WB protocol for GRK1 antibody 85942-1-RRDownload protocol
IHC protocol for GRK1 antibody 85942-1-RRDownload protocol
IF protocol for GRK1 antibody 85942-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols