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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRK1 Recombinant antibody (85942-1-RR)

    WB analysis using 85942-1-RR (same clone as 85942-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85942-1-RR (GRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85942-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse retina tissue using GRK1 Recombinant antibody (85942-1-RR)

    WB analysis of mouse retina using 85942-1-RR (same clone as 85942-1-PBS)

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 85942-1-RR (GRK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85942-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using GRK1 Recombinant antibody (85942-1-RR)

    IHC staining of mouse eye using 85942-1-RR (same clone as 85942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85942-1-RR (GRK1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85942-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using GRK1 Recombinant antibody (85942-1-RR)

    IHC staining of mouse eye using 85942-1-RR (same clone as 85942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85942-1-RR (GRK1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85942-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using GRK1 Recombinant antibody (85942-1-RR)

    IF Staining of mouse eye using 85942-1-RR (same clone as 85942-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using GRK1 antibody (85942-1-RR, Clone: 250433A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85942-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85942-1-RR

    Affinity and Kinetic Characterization of 85942-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85942-1-RR against Human GRK1 were performed. The affinity constant is 3.77 nM.

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GRK1 Recombinant antibody, PBS Only

GRK1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85942-1-PBS
Clone No.250433A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

EC:2.7.11.14, G protein-coupled receptor kinase 1, GPRK1, Rhodopsin kinase, Rhodopsin kinase GRK1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85942-1-PBS targets GRK1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRK1 fusion protein Ag18063 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor kinase 1
Calculated molecular weight 563 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC140945
Gene Symbol GRK1
Gene ID (NCBI) 6011
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15835
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1, and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in the phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.