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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using GRHPR Polyclonal antibody (51013-2-AP)

    WB analysis of mouse liver using 51013-2-AP (same clone as 51013-2-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51013-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using GRHPR Polyclonal antibody (51013-2-AP)

    WB analysis of L02 using 51013-2-AP (same clone as 51013-2-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51013-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using GRHPR Polyclonal antibody (51013-2-AP)

    WB analysis of human liver using 51013-2-AP (same clone as 51013-2-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51013-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using GRHPR Polyclonal antibody (51013-2-AP)

    WB analysis of MCF-7 using 51013-2-AP (same clone as 51013-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51013-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GRHPR Polyclonal antibody (51013-2-AP)

    IHC staining of human liver using 51013-2-AP (same clone as 51013-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 51013-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GRHPR Polyclonal antibody (51013-2-AP)

    IHC staining of human liver using 51013-2-AP (same clone as 51013-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 51013-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GRHPR Polyclonal antibody (51013-2-AP)

    IHC staining of human ovary tumor using 51013-2-AP (same clone as 51013-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51013-2-AP (GRHPR antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51013-2-PBS in a different storage buffer formulation.

GRHPR Polyclonal antibody, PBS Only

GRHPR Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 51013-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51013-2-PBS targets GRHPR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0484

Product name: Recombinant human GRHPR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-328 aa of BC000605

Sequence: VGYTPDVLTDTTAELAVSLLLTTCRRLPEAIEEVKNGGWTSWKPLWLCGYGLTQSTVGIIGLGRIGQAIARRLKPFGVQRFLYTGRQPRPEEAAEFQAEFVSTPELAAQSDFIVVACSLTPATEGLCNKDFFQKMKETAVFINISRGDVVNQDDLYQALASGKIAAAGLDVTSPEPLPTNHPLLTLKNCVILPHIGSATHRTRNTMSLLAANNLLAGLRGEPMPSELKL

相同性解析による交差性が予測される生物種
Full Name glyoxylate reductase/hydroxypyruvate reductase
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000605
Gene Symbol GRHPR
Gene ID (NCBI) 9380
RRIDAB_2113424
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBQ7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GRHPR(Glyoxylate reductase/hydroxypyruvate reductase) is also named as GLXR and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The GRHPR gene encodes a predicted 328-amino acid protein with a calculated molecular mass of 35.5 kD. It has glyoxylate reductase (GR), hydroxypyruvate reductase (HPR) and D-glycerate dehydrogenase (DGDH) activities(PMID:16597637). Defects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2)(PMID: 10484776). It can exsit as a homodimer(PMID:16756993).

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