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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRHPR Polyclonal antibody

GRHPR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51013-2-AP

Synonyms

GLXR, GLYD, GRHPR, PH2

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 51013-2-AP

    WB analysis of mouse liver using 51013-2-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 51013-2-AP

    WB analysis of human liver using 51013-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 51013-2-AP

    WB analysis of MCF-7 using 51013-2-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 51013-2-AP

    WB analysis of L02 using 51013-2-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 51013-2-AP

    IHC staining of human liver using 51013-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with GRHPR antibody included
  • IHC staining of human liver using 51013-2-AP

    IHC staining of human liver using 51013-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with GRHPR antibody included
  • IHC staining of human ovary tumor using 51013-2-AP

    IHC staining of human ovary tumor using 51013-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51013-2-AP (GRHPR antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with GRHPR antibody included

Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, L02 cells, MCF-7 cells
Positive IHC detected inhuman liver tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GRHPR Ready-To-Use IHC Kit. GRHPR primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

51013-2-AP targets GRHPR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRHPR fusion protein Ag0484 相同性解析による交差性が予測される生物種
Full Name glyoxylate reductase/hydroxypyruvate reductase
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000605
Gene symbol GRHPR
Gene ID (NCBI) 9380
RRIDAB_2113424
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GRHPR(Glyoxylate reductase/hydroxypyruvate reductase) is also named as GLXR and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The GRHPR gene encodes a predicted 328-amino acid protein with a calculated molecular mass of 35.5 kD. It has glyoxylate reductase (GR), hydroxypyruvate reductase (HPR) and D-glycerate dehydrogenase (DGDH) activities(PMID:16597637). Defects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2)(PMID: 10484776). It can exsit as a homodimer(PMID:16756993).

Protocols

Product Specific Protocols
WB protocol for GRHPR antibody 51013-2-APDownload protocol
IHC protocol for GRHPR antibody 51013-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols