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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using GRHPR Polyclonal antibody (51013-2-AP)

    WB analysis of mouse liver using 51013-2-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using GRHPR Polyclonal antibody (51013-2-AP)

    WB analysis of human liver using 51013-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GRHPR Polyclonal antibody (51013-2-AP)

    WB analysis of MCF-7 using 51013-2-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using GRHPR Polyclonal antibody (51013-2-AP)

    WB analysis of L02 using 51013-2-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using GRHPR Polyclonal antibody (51013-2-AP)

    IHC staining of human liver using 51013-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GRHPR Polyclonal antibody (51013-2-AP)

    IHC staining of human liver using 51013-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GRHPR Polyclonal antibody (51013-2-AP)

    IHC staining of human ovary tumor using 51013-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51013-2-AP (GRHPR antibody) at dilution of 1:50 (under 10x lens).

GRHPR Polyclonal antibody

GRHPR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51013-2-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

GLXR, GLYD, GRHPR, PH2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, L02 cells, MCF-7 cells
Positive IHC detected inhuman liver tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51013-2-AP targets GRHPR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0484

Product name: Recombinant human GRHPR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-328 aa of BC000605

Sequence: VGYTPDVLTDTTAELAVSLLLTTCRRLPEAIEEVKNGGWTSWKPLWLCGYGLTQSTVGIIGLGRIGQAIARRLKPFGVQRFLYTGRQPRPEEAAEFQAEFVSTPELAAQSDFIVVACSLTPATEGLCNKDFFQKMKETAVFINISRGDVVNQDDLYQALASGKIAAAGLDVTSPEPLPTNHPLLTLKNCVILPHIGSATHRTRNTMSLLAANNLLAGLRGEPMPSELKL

相同性解析による交差性が予測される生物種
Full Name glyoxylate reductase/hydroxypyruvate reductase
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000605
Gene Symbol GRHPR
Gene ID (NCBI) 9380
RRIDAB_2113424
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBQ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GRHPR(Glyoxylate reductase/hydroxypyruvate reductase) is also named as GLXR and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The GRHPR gene encodes a predicted 328-amino acid protein with a calculated molecular mass of 35.5 kD. It has glyoxylate reductase (GR), hydroxypyruvate reductase (HPR) and D-glycerate dehydrogenase (DGDH) activities(PMID:16597637). Defects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2)(PMID: 10484776). It can exsit as a homodimer(PMID:16756993).

Protocols

Product Specific Protocols
IHC protocol for GRHPR antibody 51013-2-APDownload protocol
WB protocol for GRHPR antibody 51013-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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