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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CoraLite® Plus 488-conjugated GREM2 Polyclonal ant (CL488-13892)

    IF Staining of mouse kidney using CL488-13892

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite® Plus 488 GREM2 antibody (CL488-13892) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HCT 116 cells using CoraLite® Plus 488-conjugated GREM2 Polyclonal ant (CL488-13892)

    FC experiment of HCT 116 using CL488-13892

    1X10^6 HCT 116 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GREM2 (CL488-13892) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated GREM2 Polyclonal antibody

GREM2 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-13892

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Synonyms

CKTSF1B2, DAND 3, DAND3, GREM 2, Gremlin 2

Formulation:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse kidney tissue
Positive FC (Intra) detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13892 targets GREM2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4854

Product name: Recombinant human GREM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-168 aa of BC046632

Sequence: AIHSPYKDGSSNNSERWQHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQTVSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEESFQSCAFCKPQRVTSVLVELECPGLDPPFRLKKIQKVKQCRCMSVNLSDSDKQ

相同性解析による交差性が予測される生物種
Full Name gremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)
Calculated molecular weight 168 aa, 19 kDa
GenBank accession numberBC046632
Gene Symbol GREM2
Gene ID (NCBI) 64388
RRIDAB_3083948
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H772
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 GREM2 antibody CL488-13892Download protocol
IF protocol for CL Plus 488 GREM2 antibody CL488-13892Download protocol
Standard Protocols
Click here to view our Standard Protocols
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