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Validation Data Gallery

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  • Flow cytometry (FC) experiment of RAW 264.7 cells using FITC-conjugated GPR65 Polyclonal antibody (FITC-20306)

    FC experiment of RAW 264.7 using FITC-20306

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.8 ug FITC-conjugated GPR65 Polyclonal antibody (FITC-20306)(red), or 0.8 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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FITC-conjugated GPR65 Polyclonal antibody

GPR65 Polyclonal Antibody for ELISA
Cat No. FITC-20306

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA

Synonyms

G protein coupled receptor 65, G-protein coupled receptor 65, hTDAG8, Psychosine receptor, T-cell death-associated gene 8 protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  FITC
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-20306 targets GPR65 in ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR65 fusion protein Ag14139 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 65
Calculated molecular weight 337 aa, 39 kDa
GenBank accession numberBC035633
Gene Symbol GPR65
Gene ID (NCBI) 8477
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths498 nm / 526 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYL9
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR65 (G protein-coupled receptor 65) was defined originally as T-cell death-associated gene 8 (TDAG8), functions as a proton-sensing receptor, and is also sensitive to psychosine (PMID: 35343079). GPR65 promotes adaptation to an acidic environment to enhance cell survival and proliferation, thereby promoting tumor development (PMID: 36852075).

Protocols

Product Specific Protocols
FC protocol for FITC GPR65 antibody FITC-20306Download protocol
Standard Protocols
Click here to view our Standard Protocols