Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
28232-1-PBS targets GPR4 in IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28273 Product name: Recombinant human GPR4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 203-362 aa of BC067535 Sequence: GILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 4 |
| Calculated molecular weight | 362 aa, 41 kDa |
| GenBank accession number | BC067535 |
| Gene Symbol | GPR4 |
| Gene ID (NCBI) | 2828 |
| RRID | AB_3086039 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46093 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
GPR4 (G-protein-coupled receptor 4) is a proton-sensing G-protein-coupled receptor (GPCR) that contributes to neovascularization in acidic microenvironments (PMID: 33632325). GPR4 is highly expressed in vascular endothelial cells (ECs) and blood vessel-rich tissues such as the lung, kidney, heart, and liver. Moreover, GPR4 has been shown to mediate EC inflammatory responses to acidosis and is central for leukocyte-endothelium interaction (PMID: 27940273).