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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPR4 Polyclonal antibody

GPR4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 28232-1-AP

Synonyms

G protein coupled receptor 19, G protein coupled receptor 4, GPR19, GPR4

Validation Data Gallery

Applications:
  • IHC staining of mouse lung using 28232-1-AP

    IHC staining of mouse lung using 28232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 28232-1-AP

    IHC staining of mouse kidney using 28232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inmouse lung tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28232-1-AP targets GPR4 in IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR4 fusion protein Ag28273 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 4
Calculated molecular weight 362 aa, 41 kDa
GenBank accession numberBC067535
Gene symbol GPR4
Gene ID (NCBI) 2828
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR4 (G-protein-coupled receptor 4) is a proton-sensing G-protein-coupled receptor (GPCR) that contributes to neovascularization in acidic microenvironments (PMID: 33632325). GPR4 is highly expressed in vascular endothelial cells (ECs) and blood vessel-rich tissues such as the lung, kidney, heart, and liver. Moreover, GPR4 has been shown to mediate EC inflammatory responses to acidosis and is central for leukocyte-endothelium interaction (PMID: 27940273).

Protocols

Product Specific Protocols
IHC protocol for GPR4 antibody 28232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols