Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
33499-1-PBS targets GPR175 in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag39379 Product name: Recombinant human GPR175 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 286-373 aa of BC050711 Sequence: SEPKILFSYKCQVDETEEPDVHLPQPYAVARREGLEAAGAAGASAASYSSTQFDSAGGVAYLDDIASMPCHTGSINSTDSERWKAINA 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 175 |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC050711 |
| Gene Symbol | GPR175 |
| Gene ID (NCBI) | 131601 |
| RRID | AB_3742992 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86W33 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
GPR175, also known as TPRA1 or TPRA40, is a transmembrane protein. GPR175 was first cloned as a GLP-1 receptor homolog in 3T3-L1 adipocytes and is also expressed in tissues that require Hh signaling during development, including the heart, brain, lung, pancreas, and muscle. Endogenous GPR175 localizes to cilia in a Hh-dependent manner, where it can interact with (constitutively) ciliary Gαi1 to inhibit the local production of cAMP by adenylyl cyclase. The calculated molecular weight of GPR175 is 41 kDa. With glycosylation modification, the molecular weight of GPR175 will be migrated to 55 kDa. (PMID: 26451044)