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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPR175 Polyclonal antibody (33499-1-AP)

    WB analysis using 33499-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 33499-1-AP (GPR175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GPR175 Polyclonal antibody (33499-1-AP)

    WB analysis using 33499-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 33499-1-AP (GPR175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

GPR175 Polyclonal antibody

GPR175 Polyclonal Antibody for WB, ELISA
Cat No. 33499-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Integral membrane protein GPR175, PP6566, TMEM227, TPRA1, TPRA40

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33499-1-AP targets GPR175 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag39379

Product name: Recombinant human GPR175 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 286-373 aa of BC050711

Sequence: SEPKILFSYKCQVDETEEPDVHLPQPYAVARREGLEAAGAAGASAASYSSTQFDSAGGVAYLDDIASMPCHTGSINSTDSERWKAINA

相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 175
Observed molecular weight55 kDa
GenBank accession numberBC050711
Gene Symbol GPR175
Gene ID (NCBI) 131601
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86W33
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR175, also known as TPRA1 or TPRA40, is a transmembrane protein. GPR175 was first cloned as a GLP-1 receptor homolog in 3T3-L1 adipocytes and is also expressed in tissues that require Hh signaling during development, including the heart, brain, lung, pancreas, and muscle. Endogenous GPR175 localizes to cilia in a Hh-dependent manner, where it can interact with (constitutively) ciliary Gαi1 to inhibit the local production of cAMP by adenylyl cyclase. The calculated molecular weight of GPR175 is 41 kDa. With glycosylation modification, the molecular weight of GPR175 will be migrated to 55 kDa. (PMID: 26451044)

Protocols

Product Specific Protocols
WB protocol for GPR175 antibody 33499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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