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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPAT3 Polyclonal antibody (30765-1-AP)

    WB analysis using 30765-1-AP (same clone as 30765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPAT3 Polyclonal antibody (30765-1-AP)

    WB analysis using 30765-1-AP (same clone as 30765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using GPAT3 Polyclonal antibody (30765-1-AP)

    WB analysis of HT-1080 using 30765-1-AP (same clone as 30765-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GPAT3 Polyclonal antibody (30765-1-AP)

    WB analysis of HeLa using 30765-1-AP (same clone as 30765-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GPAT3 Polyclonal antibody (30765-1-AP)

    IHC staining of mouse kidney using 30765-1-AP (same clone as 30765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GPAT3 Polyclonal antibody (30765-1-AP)

    IHC staining of mouse kidney using 30765-1-AP (same clone as 30765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GPAT3 Polyclonal antibody (30765-1-AP)

    IHC staining of mouse liver using 30765-1-AP (same clone as 30765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GPAT3 Polyclonal antibody (30765-1-AP)

    IHC staining of mouse liver using 30765-1-AP (same clone as 30765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

GPAT3 Polyclonal antibody, PBS Only

GPAT3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30765-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30765-1-PBS targets GPAT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33717

Product name: Recombinant human GPAT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 35-136 aa of Glycerol-3-phosphate acyltransferase 3

Sequence: SEIYMKILVKTLEWATIRIEKGTPKESILKNSASVGIIQRDESPMEKGLSGLRGRDFELSDVFYFSKKGLEAIVEDEVTQRFSSEELVSWNLLTRTNVNFQY*

相同性解析による交差性が予測される生物種
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 9
Calculated molecular weight49 kDa
Observed molecular weight40-45 kDa
GenBank accession numberGlycerol-3-phosphate acyltransferase 3
Gene Symbol AGPAT9
Gene ID (NCBI) 84803
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53EU6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GPAT3, also known as AGPAT9, is a member of the lysophosphatidic acid acyltransferase protein family. GPAT3 is an enzyme that catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol.

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