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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPAT3 Polyclonal antibody

GPAT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30765-1-AP

Synonyms

1 AGP acyltransferase 9, 1 AGPAT 9, AGPAT8, AGPAT9, GPAT 3, GPAT3, hGPAT3, HMFN0839, LPAAT theta, MAG 1, MAG1, UNQ2753/PRO6492

Validation Data Gallery

Applications:
  • WB analysis using 30765-1-AP

    WB analysis using 30765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30765-1-AP

    WB analysis using 30765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 30765-1-AP

    IHC staining of mouse kidney using 30765-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 30765-1-AP

    IHC staining of mouse kidney using 30765-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 30765-1-AP

    IHC staining of mouse liver using 30765-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 30765-1-AP

    IHC staining of mouse liver using 30765-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, A375 cells, Jurkat cells, MKN-45 cells
Positive IHC detected inmouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30765-1-AP targets GPAT3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPAT3 fusion protein Ag33717 相同性解析による交差性が予測される生物種
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 9
Calculated molecular weight49 kDa
Observed molecular weight40-45 kDa
GenBank accession numberGlycerol-3-phosphate acyltransferase 3
Gene symbol AGPAT9
Gene ID (NCBI) 84803
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPAT3 is a member of the lysophosphatidic acid acyltransferase protein family. GPAT3 is an enzyme that catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol.

Protocols

Product Specific Protocols
WB protocol for GPAT3 antibody 30765-1-APDownload protocol
IHC protocol for GPAT3 antibody 30765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols