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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLIM4 Recombinant antibody (84818-5-RR)

    WB analysis using 84818-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84818-5-RR (GOLIM4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLIM4 Recombinant antibody (84818-5-RR)

    IF Staining of HeLa using 84818-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLIM4 antibody (84818-5-RR, Clone: 242323F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U-2 OS using GOLIM4 Recombinant antibody (84818-5-RR)

    FC experiment of U-2 OS using 84818-5-RR

    1x10^6 U-2 OS cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of HeLa cells using GOLIM4 Recombinant antibody (84818-5-RR)

    FC experiment of HeLa using 84818-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84818-5-RR

    Affinity and Kinetic Characterization of 84818-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84818-5-RR against Human GOLIM4 were performed. The affinity constant is 0.926 nM.

GOLIM4 Recombinant monoclonal antibody

GOLIM4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84818-5-RR
Clone No.242323F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

242323F4, GIMPC, GOLPH4, GPP130, P 138

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, MCF-7 cells, HT-1080 cells, L02 cells, C2C12 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-2 OS, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84818-5-RR targets GOLIM4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34962

Product name: Recombinant human GOLIM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 350-500 aa of NM_014498

Sequence: QVGQAEHLEEEHDPSPEEQDREWKEQHEQREAANLLEGHARAEVYPSAKPMIKFQSPYEEQLEQQRLAVQQVEEAQQLREHQEALHQQRLQGHLLRQQEQQQQQVAREMALQRQAELEEGRPQHQEQLRQQAHYDAMDNDIVQGAEDQGIQ

相同性解析による交差性が予測される生物種
Full Name golgi integral membrane protein 4
Calculated molecular weight82 kDa
Observed molecular weight130-140 kDa
GenBank accession numberNM_014498
Gene Symbol GOLIM4
Gene ID (NCBI) 27333
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO00461
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Golgi integral membrane protein 4 (GOLIM4) is a 130-kDa membrane protein that is integrated into the cis/medial Golgi apparatus, which is a kind II Golgi protein. GOLIM4 is a Golgi protein that circulates between Golgi apparatus and endosomes, and its shuttle is pH sensitive(PMID:30068697).

Protocols

Product Specific Protocols
IF protocol for GOLIM4 antibody 84818-5-RRDownload protocol
WB protocol for GOLIM4 antibody 84818-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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