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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLIM4 Recombinant monoclonal antibody (84818-5-RR)

    WB analysis using 84818-5-RR (same clone as 84818-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84818-5-RR (GOLIM4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84818-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLIM4 Recombinant monoclonal antibody (84818-5-RR)

    IF Staining of HeLa using 84818-5-RR (same clone as 84818-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLIM4 antibody (84818-5-RR, Clone: 242323F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 84818-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using GOLIM4 Recombinant monoclonal antibody (84818-5-RR)

    FC experiment of U-2 OS using 84818-5-RR (same clone as 84818-5-PBS)

    1x10^6 U-2 OS cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84818-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GOLIM4 Recombinant monoclonal antibody (84818-5-RR)

    FC experiment of HeLa using 84818-5-RR (same clone as 84818-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84818-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84818-5-RR

    Affinity and Kinetic Characterization of 84818-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84818-5-RR against Human GOLIM4 were performed. The affinity constant is 0.926 nM.

GOLIM4 Recombinant monoclonal antibody, PBS Only

GOLIM4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84818-5-PBS
Clone No.242323F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242323F4, GIMPC, GOLPH4, GPP130, P 138

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84818-5-PBS targets GOLIM4 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34962

Product name: Recombinant human GOLIM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 350-500 aa of NM_014498

Sequence: QVGQAEHLEEEHDPSPEEQDREWKEQHEQREAANLLEGHARAEVYPSAKPMIKFQSPYEEQLEQQRLAVQQVEEAQQLREHQEALHQQRLQGHLLRQQEQQQQQVAREMALQRQAELEEGRPQHQEQLRQQAHYDAMDNDIVQGAEDQGIQ

相同性解析による交差性が予測される生物種
Full Name golgi integral membrane protein 4
Calculated molecular weight82 kDa
Observed molecular weight130-140 kDa
GenBank accession numberNM_014498
Gene Symbol GOLIM4
Gene ID (NCBI) 27333
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO00461
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Golgi integral membrane protein 4 (GOLIM4) is a 130-kDa membrane protein that is integrated into the cis/medial Golgi apparatus, which is a kind II Golgi protein. GOLIM4 is a Golgi protein that circulates between Golgi apparatus and endosomes, and its shuttle is pH sensitive(PMID:30068697).

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