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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated GMF Beta Monoclonal antibody

GMF Beta Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B10A2

Cat no : CL488-60062

Synonyms

Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB

Validation Data Gallery

Applications:
  • IF Staining of U-87 MG using CL488-60062

    IF Staining of U-87 MG using CL488-60062

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using CoraLite® Plus 488 GMF Beta antibody (CL488-60062, Clone: 3B10A2 ) at dilution of 1:200.

Tested Applications

Positive IF detected inU-87 MG cells
For other applications, we recommend the unconjugated version of this antibody, 60062-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60062 targets GMF Beta in IF applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GMF Beta fusion protein Ag1104 相同性解析による交差性が予測される生物種
Full Name glia maturation factor, beta
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC005359
Gene symbol GMFB
Gene ID (NCBI) 2764
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GMF Beta antibody CL488-60062Download protocol
Standard Protocols
Click here to view our Standard Protocols