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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of rat pancreas tissue using GLP1R Monoclonal antibody (60931-1-Ig)

    IF Staining of rat pancreas using 60931-1-Ig

    FFPE-rat pancreas tissue was stained with GLP1R monoclonal antibody (Cat.NO. 60931-1-Ig) at 1:500 followed by detection with Multi-rAb® Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) (Cat.NO. RGAU011). CoraLite®Plus 647-Tyramide Reagent (Cat.NO. PR30025) was used for signal development (purple). The slide was then conducted HIER in pH 9.0 Tris-EDTA buffer followed by staining with INS monoclonal antibody (66198-1-Ig, 1:5000). CoraLite®Plus 488-Tyramide Reagent (Cat.NO. PR30022) were used for signal development (green). Cell nucleus (blue) were stained with DAPI. The signal from each channel was captured and merged.

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GLP1R Monoclonal antibody

GLP1R Monoclonal Antibody for IF/ICC, ELISA
Cat No. 60931-1-Ig
Clone No.1A4G5

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, ELISA

Synonyms

GLP 1 R, GLP 1 receptor, GLP 1R, GLP-1 receptor, GLP-1R

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inrat pancreas tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60931-1-Ig targets GLP1R in IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GLP1R fusion protein Ag23529 相同性解析による交差性が予測される生物種
Full Name glucagon-like peptide 1 receptor
Calculated molecular weight 463 aa, 53 kDa
GenBank accession numberBC112126
Gene Symbol GLP1R
Gene ID (NCBI) 2740
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP43220
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLP1R (glucagon-like peptide-1 receptor) is a G protein-coupled receptor belonging to the secretin receptor superfamily, also known as class B (PMID:34911028). GLP1R is widely distributed in pancreatic islets, muscles, the gastrointestinal tract, the lung, liver, pancreas, and other tissues or organs (PMID:35989517). Moreover, GLP1R agonist has been reported to induce autophagy of EC (Endometrial carcinoma) cells, and high GLP1R expression may be associated with good prognosis of EC patients, suggesting that GLP1R is likely to participate in the progression of EC (PMID:29907137). Moreover, regarding the molecular weight of GLP1R, research shows that the bands at ~50 kDa and ~100 kDa belong to the monomer and dimer states of GLP1R (PMID: 28609478).

Protocols

Product Specific Protocols
IF protocol for GLP1R antibody 60931-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols