Validation Data Gallery
Tested Applications
| Positive IHC detected in | human pancreas tissue, rat pancreas tissue, human pancreas tissue, human pancreas cancer tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human pancreas tissue, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 17 publications below |
| IF | See 58 publications below |
Product Information
66198-1-Ig targets INS in WB, IHC, IF-P, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8630 Product name: Recombinant human Insulin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-110 aa of BC005255 Sequence: MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN 相同性解析による交差性が予測される生物種 |
| Full Name | INS |
| Calculated molecular weight | 110 aa, 12 kDa |
| GenBank accession number | BC005255 |
| Gene Symbol | INS |
| Gene ID (NCBI) | 3630 |
| RRID | AB_2881591 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01308 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, pro-INS, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for INS antibody 66198-1-Ig | Download protocol |
| IHC protocol for INS antibody 66198-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology Cancer-associated endocrine cells participate in pancreatic carcinogenesis | ||
Bioact Mater Subaqueous acoustic pressure system based one day heterotypic pseudo-islet spheroid formation with adipose derived stem cells for graft survival-related function enhancement | ||
Nat Commun 5'tRNA-derived fragments modulate β-cell homeostasis and islet macrophage activation in type 2 diabetes. | ||
Nat Commun OSGEP regulates islet β-cell function by modulating proinsulin translation and maintaining ER stress homeostasis in mice | ||
Adv Sci (Weinh) CD168 Identifies Proliferating Pancreatic Islet Cells in Murine and Human. | ||
Adv Sci (Weinh) Selenium Nanodots (SENDs) as Antioxidants and Antioxidant-Prodrugs to Rescue Islet β Cells in Type 2 Diabetes Mellitus by Restoring Mitophagy and Alleviating Endoplasmic Reticulum Stress |