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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GIGYF2 Polyclonal antibody (24790-1-AP)

    WB analysis using 24790-1-AP (same clone as 24790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24790-1-AP (GIGYF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GIGYF2 Polyclonal antibody (24790-1-AP)

    WB analysis of HeLa using 24790-1-AP (same clone as 24790-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24790-1-AP (GIGYF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using GIGYF2 Polyclonal antibody (24790-1-AP)

    IP experiment of Jurkat using 24790-1-AP (same clone as 24790-1-PBS)

    IP result of anti-GIGYF2 (IP:24790-1-AP, 4ug; Detection:24790-1-AP 1:2000) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using GIGYF2 Polyclonal antibody (24790-1-AP)

    IHC staining of rat brain using 24790-1-AP (same clone as 24790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24790-1-AP (GIGYF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GIGYF2 Polyclonal antibody (24790-1-AP)

    IHC staining of mouse brain using 24790-1-AP (same clone as 24790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24790-1-AP (GIGYF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GIGYF2 Polyclonal antibody (24790-1-AP)

    IF Staining of HeLa using 24790-1-AP (same clone as 24790-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GIGYF2 antibody (24790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GIGYF2 Polyclonal antibody, PBS Only

GIGYF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24790-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

GRB10-interacting GYF protein 2, GYF2, KIAA0642, PERQ amino acid-rich with GYF domain-containing protein 2, PERQ2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24790-1-PBS targets GIGYF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20329

Product name: Recombinant human GIGYF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 777-1104 aa of BC146775

Sequence: KRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELEVQRQKELMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPSGTTKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSNADTKNSNMGFWDDAV

相同性解析による交差性が予測される生物種
Full Name GRB10 interacting GYF protein 2
Calculated molecular weight 1320 aa, 152 kDa
Observed molecular weight 150-170 kDa
GenBank accession numberBC146775
Gene Symbol GIGYF2
Gene ID (NCBI) 26058
RRIDAB_2879727
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6Y7W6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GIGYF2 (GRB10 interacting GYF protein 2) was initially identified through its interaction with GRB10, an adapter protein that binds activated INS-like growth factor (IGF-I) and INS receptors (PMID: 12771153; 19744960). It may act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. The gene of GIGYF2 has been proposed as the Parkinson-disease (PD) gene underlying the PARK11 locus (PMID: 18358451).

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