Validation Data Gallery
Product Information
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
| Description | The GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy: • Considerably higher tissue penetration rates • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments • Less than 2 nm epitope-label displacement minimizes linkage error • Monovalent VHHs do not cluster their epitopes • Validation: structure and function characterized • Consistent and reliable performance due to recombinant production |
| Applications | IF, IHC, ICC |
| Host | Alpaca |
| Specificity/Target | CFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet For the complete list, please click here: Fluorescent protein specificity table Nano-Booster |
| Conjugate | Alexa Fluor® 488 |
| Physical State | Liquid |
| Suggested Dilution | IF/ICC/IHC: 1:500- 1:1,000 |
| Type | Nanobody |
| Class | Recombinant |
| DOL | 2 fluorophores per Nanobody |
| Format | Alpaca single domain antibody, Nanobody or VHH; monovalent |
| RRID | AB_2827573 |
| Affinity (KD) | Dissociation constant KD of 1 pM |
| Excitation/ Emission | Excitation max: 490 nm, Emission max: 525 nm |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light. |
| Size | 10 μL; 50 μL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
|---|
| gb2AF488_SDS_GFP-Booster Alexa Fluor® 488 (EN) |
| Datasheet |
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| Protocol GFP-Booster Alexa Fluor 488 (PDF) |
| GFP-TRAP Specificity |
|---|
| Fluorescent protein specificity table Nano-Booster |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
|---|---|
Nat Biotechnol Three-dimensional structured illumination microscopy with enhanced axial resolution | |
Nat Cell Biol CDK1-cyclin-B1-induced kindlin degradation drives focal adhesion disassembly at mitotic entry. | |
Mol Cell Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization. | |
Nucleic Acids Res COSA-1 mediated pro-crossover complex formation promotes meiotic crossing over in C. elegans | |
J Exp Med Bhlhe40 function in activated B and TFH cells restrains the GC reaction and prevents lymphomagenesis. |