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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GFAP Recombinant antibody

GFAP Recombinant Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat , pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4C6

Cat no : 81063-1-RR

Synonyms

FLJ45472, GFAP

Validation Data Gallery

Applications:
  • WB analysis of U-251 using 81063-1-RR

    WB analysis of U-251 using 81063-1-RR

    U-251 cells were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 81063-1-RR

    WB analysis using 81063-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 81063-1-RR

    IHC staining of rat brain using 81063-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GFAP antibody included
  • IHC staining of rat brain using 81063-1-RR

    IHC staining of rat brain using 81063-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GFAP antibody included
  • IF Staining of rat brain using 81063-1-RR

    IF Staining of rat brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 81063-1-RR

    IF Staining of mouse brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 81063-1-RR

    IF Staining of mouse brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inU-251 cells, rat brain tissue, mouse brain tissue, pig brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy GFAP Ready-To-Use IHC Kit. GFAP primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4950-1:19800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

81063-1-RR targets GFAP in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat , pig samples.

Tested Reactivity Human, mouse, rat , pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GFAP fusion protein Ag10423 相同性解析による交差性が予測される生物種
Full Name glial fibrillary acidic protein
Calculated molecular weight 432 aa, 50 kDa
GenBank accession numberBC013596
Gene symbol GFAP
Gene ID (NCBI) 2670
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.

Protocols

Product Specific Protocols
WB protocol for GFAP antibody 81063-1-RRDownload protocol
IHC protocol for GFAP antibody 81063-1-RRDownload protocol
IF protocol for GFAP antibody 81063-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols