Validation Data Gallery
Tested Applications
Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Published Applications
IHC | See 1 publications below |
Product Information
81063-1-PBS targets GFAP as part of a matched antibody pair:
MP80011-1: 81063-1-PBS capture and 60190-4-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | GFAP fusion protein Ag10423 相同性解析による交差性が予測される生物種 |
Full Name | glial fibrillary acidic protein |
Calculated molecular weight | 432 aa, 50 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC013596 |
Gene Symbol | GFAP |
Gene ID (NCBI) | 2670 |
Conjugate | Unconjugated |
Form | |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P14136 |
Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -80°C. |
Background Information
GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.