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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using GCNT2 Polyclonal antibody (18118-1-AP)

    WB analysis of mouse kidney using 18118-1-AP (same clone as 18118-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18118-1-AP (GCNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GCNT2 Polyclonal antibody (18118-1-AP)

    WB analysis of HEK-293 using 18118-1-AP (same clone as 18118-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18118-1-AP (GCNT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using GCNT2 Polyclonal antibody (18118-1-AP)

    IP experiment of HEK-293 using 18118-1-AP (same clone as 18118-1-PBS)

    N/A This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCNT2 Polyclonal antibody (18118-1-AP)

    IHC staining of human breast cancer using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18118-1-AP (GCNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GCNT2 Polyclonal antibody (18118-1-AP)

    IHC staining of human liver using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18118-1-AP (GCNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GCNT2 Polyclonal antibody (18118-1-AP)

    IHC staining of human liver using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18118-1-AP (GCNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GCNT2 Polyclonal antibody (18118-1-AP)

    IHC staining of mouse liver using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18118-1-AP (GCNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using GCNT2 Polyclonal antibody (18118-1-AP)

    IHC staining of human colon using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using GCNT2 Polyclonal antibody (18118-1-AP)

    IHC staining of human colon using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

GCNT2 Polyclonal antibody, PBS Only

GCNT2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 18118-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CCAT, EC:2.4.1.150, GCNT2C, GCNT5, I-branching enzyme

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18118-1-PBS targets GCNT2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12635

Product name: Recombinant human GCNT2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 29-400 aa of BC074801

Sequence: GDPSFQRLNISDPLRLTQVCTSFINGKTRFLWKNKLMIHEKSSCKEYLTQSHYITAPLSKEEADFPLAYIMVIHHHFDTFARLFRAIYMPQNIYCVHVDEKATTEFKDAVEQLLSCFPNAFLASKMEPVVYGGISRLQADLNCIRDLSAFEVSWKYVINTCGQDFPLKTNKEIVQYLKGFKGKNITPGVLPPAHAIGRTKYVHQEHLGKELSYVIRTTALKPPPPHNLTIYFGSAYVALSREFANFVLHDPRAVDLLQWSKDTFSPDEHFWVTLNRIPGVPGSMPNASWTGNLRAIKWSDMEDRHGGCHGHYVHGICIYGNGDLKWLVNSPSLFANKFELNTYPLTVECLELRHRERTLNQSETAIQPSWYF

相同性解析による交差性が予測される生物種
Full Name glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
Calculated molecular weight 400 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC074801
Gene Symbol GCNT2
Gene ID (NCBI) 2651
RRIDAB_2232115
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0V5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GCNT2 (N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase) catalyzes the transfer of N-acetylglucosamine to the carbon-6 position of galactose. The loss of GCNT2 expression and corresponding loss of I-antigen in melanoma cells has profound effects on key oncogenic signaling pathways, including integrin-mediated signaling pathways critical for metastasis (PMID: 33660254, 30135430). Alterations in GCNT2 expression have now been associated with multiple malignancies, with high GCNT2 promoting tumor progression in a majority of investigated cancers. Three GCNT2 splicing variants GCNT2A, -B, and -C, which differ at exon 1 but have identical exon 2 and 3 coding regions, are expressed differentially in specific tissues (PMID: 15161861). GCNT2C determines the expression of the blood group I antigen in erythrocytes.

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