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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GCNT2 Polyclonal antibody

GCNT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18118-1-AP

Synonyms

CCAT, GCNT2, GCNT2C, GCNT5, I branching enzyme, IGNT, II, NACGT1, NAGCT1, ULG3

Validation Data Gallery

Applications:
  • WB analysis of mouse kidney using 18118-1-AP

    WB analysis of mouse kidney using 18118-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18118-1-AP (GCNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 18118-1-AP

    WB analysis of HEK-293 using 18118-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18118-1-AP (GCNT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 18118-1-AP

    IHC staining of human colon using 18118-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with GCNT2 antibody included
  • IHC staining of human colon using 18118-1-AP

    IHC staining of human colon using 18118-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with GCNT2 antibody included
  • IHC staining of human colon using 18118-1-AP

    IHC staining of human colon using 18118-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with GCNT2 antibody included
  • IHC staining of human colon using 18118-1-AP

    IHC staining of human colon using 18118-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18118-1-AP (GCNT2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with GCNT2 antibody included

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GCNT2 Ready-To-Use IHC Kit. GCNT2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

18118-1-AP targets GCNT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCNT2 fusion protein Ag12635 相同性解析による交差性が予測される生物種
Full Name glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
Calculated molecular weight 400 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC074801
Gene symbol GCNT2
Gene ID (NCBI) 2651
RRIDAB_2232115
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GCNT2 antibody 18118-1-APDownload protocol
IHC protocol for GCNT2 antibody 18118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Glycobiology

MeCP2 regulated glycogenes contribute to proliferation and apoptosis of gastric cancer cells.

Authors - Yannan Qin
View Article