Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, A549 cells, RT-4 cells, Jurkat cells, Raji cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88062-1-RR targets GCLC in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3252 Product name: Recombinant human GCLC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-252 aa of BC022487 Sequence: MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV 相同性解析による交差性が予測される生物種 |
| Full Name | glutamate-cysteine ligase, catalytic subunit |
| Calculated molecular weight | 637 aa, 73 kDa |
| Observed molecular weight | 73 kDa |
| GenBank accession number | BC022487 |
| Gene Symbol | GCLC |
| Gene ID (NCBI) | 2729 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P48506 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family. It is also named as GLCL and GLCLC. It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis. GCLC may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GCLC antibody 88062-1-RR | Download protocol |
| WB protocol for GCLC antibody 88062-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |