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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCLC Recombinant monoclonal antibody (88062-1-RR)

    WB analysis using 88062-1-RR (same clone as 88062-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88062-1-RR (GCLC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88062-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GCLC Recombinant monoclonal antibody (88062-1-RR)

    WB analysis using 88062-1-RR (same clone as 88062-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88062-1-RR (GCLC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88062-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GCLC Recombinant monoclonal antibody (88062-1-RR)

    IHC staining of human kidney using 88062-1-RR (same clone as 88062-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 88062-1-RR (GCLC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88062-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GCLC Recombinant monoclonal antibody (88062-1-RR)

    IHC staining of human kidney using 88062-1-RR (same clone as 88062-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 88062-1-RR (GCLC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88062-1-PBS in a different storage buffer formulation.

GCLC Recombinant monoclonal antibody, PBS Only

GCLC Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 88062-1-PBS
Clone No.260214H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:6.3.2.2, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88062-1-PBS targets GCLC in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3252

Product name: Recombinant human GCLC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-252 aa of BC022487

Sequence: MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV

相同性解析による交差性が予測される生物種
Full Name glutamate-cysteine ligase, catalytic subunit
Calculated molecular weight 637 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC022487
Gene Symbol GCLC
Gene ID (NCBI) 2729
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP48506
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family. It is also named as GLCL and GLCLC. It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis. GCLC may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).

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