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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBX2 Monoclonal antibody (61029-1-Ig)

    WB analysis using 61029-1-Ig (same clone as 61029-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 61029-1-Ig (GBX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using GBX2 Monoclonal antibody (61029-1-Ig)

    WB analysis of PC-3 using 61029-1-Ig (same clone as 61029-1-PBS)

    WB result of GBX2 antibody (61029-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected PC-3 cells. This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C2C12 cells using GBX2 Monoclonal antibody (61029-1-Ig)

    WB analysis of C2C12 using 61029-1-Ig (same clone as 61029-1-PBS)

    C2C12 cells were subjected to SDS PAGE followed by western blot with 61029-1-Ig (GBX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GBX2 Monoclonal antibody (61029-1-Ig)

    IHC staining of mouse brain using 61029-1-Ig (same clone as 61029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 61029-1-Ig (GBX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GBX2 Monoclonal antibody (61029-1-Ig)

    IHC staining of mouse brain using 61029-1-Ig (same clone as 61029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 61029-1-Ig (GBX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBX2 Monoclonal antibody (61029-1-Ig)

    IF Staining of HeLa using 61029-1-Ig (same clone as 61029-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBX2 antibody (61029-1-Ig, Clone: 4F3E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

GBX2 Monoclonal antibody, PBS Only

GBX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 61029-1-PBS
Clone No.4F3E4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Gastrulation and brain-specific homeobox protein 2, Homeobox protein GBX-2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

61029-1-PBS targets GBX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17109

Product name: Recombinant human GBX2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 65-248 aa of BC137448

Sequence: LPQAALQPALPPAHPHHQIPSLPTGFCSSLAQGMALTSTLMATLPGGFSASPQHQEAAAARKFAPQPLPGGGNFDKAEALQADAEDGKGFLAKEGSLLAFSAAETVQASLVGAVRGQGKDESKVEDDPKGKEESFSLESDVDYSSDDNLTGQAAHKEEDPGHALEETPPSSGAAGSTTSTGKNR

相同性解析による交差性が予測される生物種
Full Name gastrulation brain homeobox 2
Calculated molecular weight 348 aa, 37 kDa
Observed molecular weight37 kDa
GenBank accession numberBC137448
Gene Symbol GBX2
Gene ID (NCBI) 2637
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP52951
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Gastrulation Brain Homeobox 2 (GBX2) is a transcription factor belonging to the homeobox gene family, which plays a critical role in embryonic development, particularly in brain patterning and neural differentiation. It is highly conserved across vertebrates and is essential for the proper formation of the midbrain-hindbrain boundary and the development of the cerebellum. GBX2 is expressed in specific regions of the developing central nervous system and regulates downstream target genes involved in cell fate determination and tissue morphogenesis. Dysregulation or mutations in GBX2 have been associated with developmental disorders and may contribute to tumorigenesis in certain cancers. (PMID: 11063941; PMID: 31758726)

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