Validation Data Gallery
Tested Applications
| Positive WB detected in | NCI-H1299 cells, C2C12 cells, PC-3 cells, HCT 116 cells, A172 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
61029-1-Ig targets GBX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17109 Product name: Recombinant human GBX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 65-248 aa of BC137448 Sequence: LPQAALQPALPPAHPHHQIPSLPTGFCSSLAQGMALTSTLMATLPGGFSASPQHQEAAAARKFAPQPLPGGGNFDKAEALQADAEDGKGFLAKEGSLLAFSAAETVQASLVGAVRGQGKDESKVEDDPKGKEESFSLESDVDYSSDDNLTGQAAHKEEDPGHALEETPPSSGAAGSTTSTGKNR 相同性解析による交差性が予測される生物種 |
| Full Name | gastrulation brain homeobox 2 |
| Calculated molecular weight | 348 aa, 37 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC137448 |
| Gene Symbol | GBX2 |
| Gene ID (NCBI) | 2637 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P52951 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Gastrulation Brain Homeobox 2 (GBX2) is a transcription factor belonging to the homeobox gene family, which plays a critical role in embryonic development, particularly in brain patterning and neural differentiation. It is highly conserved across vertebrates and is essential for the proper formation of the midbrain-hindbrain boundary and the development of the cerebellum. GBX2 is expressed in specific regions of the developing central nervous system and regulates downstream target genes involved in cell fate determination and tissue morphogenesis. Dysregulation or mutations in GBX2 have been associated with developmental disorders and may contribute to tumorigenesis in certain cancers. (PMID: 11063941; PMID: 31758726)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GBX2 antibody 61029-1-Ig | Download protocol |
| IHC protocol for GBX2 antibody 61029-1-Ig | Download protocol |
| WB protocol for GBX2 antibody 61029-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |