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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBA Polyclonal antibody (27972-1-AP)

    WB analysis using 27972-1-AP (same clone as 27972-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27972-1-AP (GBA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27972-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using GBA Polyclonal antibody (27972-1-AP)

    WB analysis of SH-SY5Y using 27972-1-AP (same clone as 27972-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27972-1-AP (GBA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27972-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GBA Polyclonal antibody (27972-1-AP)

    IHC staining of mouse brain using 27972-1-AP (same clone as 27972-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27972-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GBA Polyclonal antibody (27972-1-AP)

    IHC staining of mouse brain using 27972-1-AP (same clone as 27972-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27972-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GBA Polyclonal antibody (27972-1-AP)

    IHC staining of mouse kidney using 27972-1-AP (same clone as 27972-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27972-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GBA Polyclonal antibody (27972-1-AP)

    IHC staining of mouse kidney using 27972-1-AP (same clone as 27972-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27972-1-PBS in a different storage buffer formulation.

GBA Polyclonal antibody, PBS Only

GBA Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27972-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

GBA1, Cholesteryl-beta-glucosidase, Cholesterol glucosyltransferase, Beta-glucosylceramidase 1, Beta-glucocerebrosidase

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27972-1-PBS targets GBA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27388

Product name: Recombinant human GBA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 75-305 aa of BC003356

Sequence: TFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGP

相同性解析による交差性が予測される生物種
Full Name glucosidase, beta; acid (includes glucosylceramidase)
Calculated molecular weight 60 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC003356
Gene Symbol GBA
Gene ID (NCBI) 2629
RRIDAB_2881024
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP04062
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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