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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GBA Polyclonal antibody

GBA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27972-1-AP

Synonyms

Acid beta glucosidase, Alglucerase, Beta glucocerebrosidase, GBA, GBA1, GC, GCB, GLUC, Glucosylceramidase, Imiglucerase

Validation Data Gallery

Applications:
  • WB analysis using 27972-1-AP

    WB analysis using 27972-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27972-1-AP (GBA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 27972-1-AP

    WB analysis of SH-SY5Y using 27972-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27972-1-AP (GBA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27972-1-AP

    IHC staining of mouse brain using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27972-1-AP

    IHC staining of mouse brain using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 27972-1-AP

    IHC staining of mouse kidney using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 27972-1-AP

    IHC staining of mouse kidney using 27972-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27972-1-AP (GBA antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells
Positive IHC detected inmouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

27972-1-AP targets GBA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBA fusion protein Ag27388 相同性解析による交差性が予測される生物種
Full Name glucosidase, beta; acid (includes glucosylceramidase)
Calculated molecular weight 60 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC003356
Gene symbol GBA
Gene ID (NCBI) 2629
RRIDAB_2881024
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GBA antibody 27972-1-APDownload protocol
IHC protocol for GBA antibody 27972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins

Authors - Fatema Akter
View Article
mouseWB

Sci Total Environ

Manganese disrupts the maturation and degradation of axonal autophagosome leading to hippocampal synaptic toxicity in mice via the activation of LRRK2 on phosphorylation of Rab10

Authors - Zhuo Ma
View Article