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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GATA3 Recombinant antibody (83808-1-RR)

    WB analysis using 83808-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GATA3 Recombinant antibody (83808-1-RR)

    WB analysis of MCF-7 using 83808-1-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using GATA3 Recombinant antibody (83808-1-RR)

    FC experiment of Jurkat using 83808-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of U937 using GATA3 Recombinant antibody (83808-1-RR)

    FC experiment of U937 using 83808-1-RR

    1x10^6 U937 were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83808-1-RR

    Affinity and Kinetic Characterization of 83808-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83808-1-RR against Human GATA3 were performed. The affinity constant is 0.686 nM.

GATA3 Recombinant monoclonal antibody

GATA3 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83808-1-RR
Clone No.240684A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684A2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inEL-4 cells, MCF-7 cells, SH-SY5Y cells, T-47D cells
Positive FC (Intra) detected inJurkat cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83808-1-RR targets GATA3 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0174

Product name: Recombinant human GATA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 224-442 aa of BC003070

Sequence: PYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCGATSTPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKEGIQTRNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSSHMLTTPTPMHPPSSLSFGPHHPSSMVTAM

相同性解析による交差性が予測される生物種
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
Observed molecular weight 37 kDa and 48 kDa
GenBank accession numberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
RRIDAB_3671395
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP23771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Trans-acting T-cell-specific transcription factor, also known as GATA3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin, and mammary gland (PMID: 25148023). GATA3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full-length GATA3 protein contains 444 aa corresponding to the molecular weights of 48 kDa, and the truncated protein was detected at approximately 37 kDa (PMID: 24758297).

Protocols

Product Specific Protocols
WB protocol for GATA3 antibody 83808-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols