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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GATA3 Recombinant monoclonal antibody (83808-1-RR)

    WB analysis using 83808-1-RR (same clone as 83808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using GATA3 Recombinant monoclonal antibody (83808-1-RR)

    WB analysis of MCF-7 using 83808-1-RR (same clone as 83808-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP01933-1

    Sandwich ELISA sample test of MP01933-1

    The mean GATA3 concentration was determined to be 21.82 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load.

  • Sandwich ELISA standard curve of MP01933-1

    Sandwich ELISA standard curve of MP01933-1

    Sandwich ELISA standard curve of MP01933-1, Human GATA3 Recombinant Matched Antibody Pair - PBS only. 83808-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0174. 83808-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GATA3 Recombinant monoclonal antibody (83808-1-RR)

    IF Staining of MCF-7 using 83808-1-RR (same clone as 83808-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (83808-1-RR, Clone: 240684A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using GATA3 Recombinant monoclonal antibody (83808-1-RR)

    FC experiment of Jurkat using 83808-1-RR (same clone as 83808-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U937 using GATA3 Recombinant monoclonal antibody (83808-1-RR)

    FC experiment of U937 using 83808-1-RR (same clone as 83808-1-PBS)

    1x10^6 U937 were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01933-1

    Cytometric bead array standard curve of MP01933-1

    Cytometric bead array standard curve of MP01933-1, GATA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83808-1-PBS. Detection antibody: 83808-4-PBS. Standard: Ag0174. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83808-1-RR

    Affinity and Kinetic Characterization of 83808-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83808-1-RR against Human GATA3 were performed. The affinity constant is 0.686 nM.

GATA3 Recombinant monoclonal antibody, PBS Only (Capture)

GATA3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 83808-1-PBS
Clone No.240684A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

240684A2, GATA binding factor 3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83808-1-PBS targets GATA3 as part of a matched antibody pair:

MP01933-1: 83808-1-PBS capture and 83808-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0174

Product name: Recombinant human GATA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 224-442 aa of BC003070

Sequence: PYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCGATSTPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKEGIQTRNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSSHMLTTPTPMHPPSSLSFGPHHPSSMVTAM

相同性解析による交差性が予測される生物種
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
Observed molecular weight 37 kDa and 48 kDa
GenBank accession numberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
RRIDAB_3671395
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP23771
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Trans-acting T-cell-specific transcription factor, also known as GATA3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin, and mammary gland (PMID: 25148023). GATA3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full-length GATA3 protein contains 444 aa corresponding to the molecular weights of 48 kDa, and the truncated protein was detected at approximately 37 kDa (PMID: 24758297).

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