Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-67939 targets GART in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30509 Product name: Recombinant human GART protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-318 aa of BC038958 Sequence: MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLTKNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLD 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase |
| Calculated molecular weight | 1010 aa, 108 kDa |
| Observed molecular weight | 46 kDa, 108 kDa |
| GenBank accession number | BC038958 |
| Gene Symbol | GART |
| Gene ID (NCBI) | 2618 |
| RRID | AB_2934787 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P22102 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GART antibody CL594-67939 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
