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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAR1 Recombinant antibody (83577-4-RR)

    WB analysis using 83577-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83577-4-RR (GAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of K562 using GAR1 Recombinant antibody (83577-4-RR)

    FC experiment of K562 using 83577-4-RR

    1x10^6 K562 were intracellularly stained with 0.2 ug GAR1 Recombinant antibody (83577-4-RR, Clone:240537A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83577-4-RR

    Affinity and Kinetic Characterization of 83577-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83577-4-RR against Human GAR1 were performed. The affinity constant is 1.18 nM.

GAR1 Recombinant monoclonal antibody

GAR1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83577-4-RR
Clone No.240537A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

snoRNP protein GAR1, NOLA1, 240537A2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inA375 cells, HeLa cells, mouse skin tissue
Positive FC (Intra) detected inK562

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83577-4-RR targets GAR1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2282

Product name: Recombinant human GAR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-217 aa of BC003413

Sequence: MSFRGGGRGGFNRGGGGGGFNRGGSSNHFRGGGGGGGGGNFRGGGRGGFGRGGGRGGFNKGQDQGPPERVVLLGEFLHPCEDDIVCKCTTDENKVPYFNAPVYLENKEQIGKVDEIFGQLRDFYFSVKLSENMKASSFKKLQKFYIDPYKLLPLQRFLPRPPGEKGPPRGGGRGGRGGGRGGGGRGGGRGGGFRGGRGGGGGGFRGGRGGGFRGRGH

相同性解析による交差性が予測される生物種
Full Name GAR1 ribonucleoprotein homolog (yeast)
Calculated molecular weight 217 aa, 22 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC003413
Gene Symbol GAR1
Gene ID (NCBI) 54433
RRIDAB_3671189
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NY12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.

Protocols

Product Specific Protocols
WB protocol for GAR1 antibody 83577-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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