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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAR1 Recombinant antibody (83577-4-RR)

    WB analysis using 83577-4-RR (same clone as 83577-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83577-4-RR (GAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83577-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K562 using GAR1 Recombinant antibody (83577-4-RR)

    FC experiment of K562 using 83577-4-RR (same clone as 83577-4-PBS)

    1x10^6 K562 were intracellularly stained with 0.2 ug GAR1 Recombinant antibody (83577-4-RR, Clone:240537A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83577-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83577-4-RR

    Affinity and Kinetic Characterization of 83577-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83577-4-RR against Human GAR1 were performed. The affinity constant is 1.18 nM.

GAR1 Recombinant monoclonal antibody, PBS Only

GAR1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83577-4-PBS
Clone No.240537A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

snoRNP protein GAR1, NOLA1, 240537A2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83577-4-PBS targets GAR1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2282

Product name: Recombinant human GAR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-217 aa of BC003413

Sequence: MSFRGGGRGGFNRGGGGGGFNRGGSSNHFRGGGGGGGGGNFRGGGRGGFGRGGGRGGFNKGQDQGPPERVVLLGEFLHPCEDDIVCKCTTDENKVPYFNAPVYLENKEQIGKVDEIFGQLRDFYFSVKLSENMKASSFKKLQKFYIDPYKLLPLQRFLPRPPGEKGPPRGGGRGGRGGGRGGGGRGGGRGGGFRGGRGGGGGGFRGGRGGGFRGRGH

相同性解析による交差性が予測される生物種
Full Name GAR1 ribonucleoprotein homolog (yeast)
Calculated molecular weight 217 aa, 22 kDa
GenBank accession numberBC003413
Gene Symbol GAR1
Gene ID (NCBI) 54433
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NY12
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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