Validation Data Gallery
Tested Applications
| Positive WB detected in | unboiled rat brain tissue, unboiled mouse brain tissue, mouse cerebellum tissue |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-83057-2 targets GABRA2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag29301 Product name: Recombinant human GABRA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 348-412 aa of NM_000807 Sequence: SVVNDKKKEKASVMIQNNAYAVAVANYAPNLSKDPVLSTISKSATTPEPNKKPENKPAEAKKTFN 相同性解析による交差性が予測される生物種 |
| Full Name | gamma-aminobutyric acid (GABA) A receptor, alpha 2 |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 50-51 kDa |
| GenBank accession number | NM_000807 |
| Gene Symbol | GABRA2 |
| Gene ID (NCBI) | 2555 |
| RRID | AB_3673764 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P47869 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 GABRA2 antibody CL750-83057-2 | Download protocol |
| WB protocol for CL Plus 750 GABRA2 antibody CL750-83057-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |