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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Foxp3 Recombinant antibody (85128-6-RR)

    WB analysis using 85128-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85128-6-RR (Foxp3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    WB analysis using 85128-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85128-6-RR (Foxp3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Foxp3 Recombinant antibody (85128-6-RR)

    IHC staining of mouse spleen using 85128-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Foxp3 Recombinant antibody (85128-6-RR)

    IHC staining of human tonsillitis using 85128-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Foxp3 Recombinant antibody (85128-6-RR)

    IHC staining of mouse spleen using 85128-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    IHC staining of mouse spleen using 85128-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    IHC staining of mouse spleen using 85128-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Foxp3 Recombinant antibody (85128-6-RR)

    IF Staining of mouse spleen using 85128-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Foxp3 antibody (85128-6-RR, Clone: 242853G11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Foxp3 Recombinant antibody (85128-6-RR)

    IF Staining of mouse spleen using 85128-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Foxp3 antibody (85128-6-RR, Clone: 242853G11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Foxp3 Recombinant monoclonal antibody

Foxp3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85128-6-RR
Clone No.242853G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

forkhead box P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, JM2, scurfin

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse thymus tissue, MJ cells, rat thymus tissue, mouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85128-6-RR targets Foxp3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg4360

Product name: Recombinant Mouse Foxp3 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10007378 of

Sequence: YPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPS

相同性解析による交差性が予測される生物種
Full Name forkhead box P3
Observed molecular weight 45 kDa
Gene Symbol Foxp3
Gene ID (NCBI) 20371
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ99JB6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. FOXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome.

Protocols

Product Specific Protocols
IF protocol for Foxp3 antibody 85128-6-RRDownload protocol
IHC protocol for Foxp3 antibody 85128-6-RRDownload protocol
WB protocol for Foxp3 antibody 85128-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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