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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    WB analysis using 85128-6-RR (same clone as 85128-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85128-6-RR (Foxp3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    WB analysis using 85128-6-RR (same clone as 85128-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85128-6-RR (Foxp3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    IHC staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    IHC staining of human tonsillitis using 85128-6-RR (same clone as 85128-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    IHC staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    IHC staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    IHC staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85128-6-RR (Foxp3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    IF Staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Foxp3 antibody (85128-6-RR, Clone: 242853G11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Foxp3 Recombinant monoclonal antibody (85128-6-RR)

    IF Staining of mouse spleen using 85128-6-RR (same clone as 85128-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Foxp3 antibody (85128-6-RR, Clone: 242853G11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85128-6-PBS in a different storage buffer formulation.

Mouse Foxp3 Recombinant monoclonal antibody, PBS Only

Foxp3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85128-6-PBS
Clone No.242853G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

forkhead box P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, JM2, scurfin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85128-6-PBS targets Foxp3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg4360

Product name: Recombinant Mouse Foxp3 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10007378 of

Sequence: YPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPS

相同性解析による交差性が予測される生物種
Full Name forkhead box P3
Observed molecular weight 45 kDa
Gene Symbol Foxp3
Gene ID (NCBI) 20371
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ99JB6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. FOXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome.

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