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Validation Data Gallery

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  • Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, orange). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, orange). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, yellow). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, yellow). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with CoraLite® 488-conjugated Tom20 antibody (CL488-66777, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti- PAF49 antibody labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with CoraLite® 488-conjugated Tom20 antibody (CL488-66777, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti- PAF49 antibody labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Actin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-Lamin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Actin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-Lamin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062).

    Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062).

  • Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062).​

    Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062).​

  • WB of HeLa and HEK-293 cell lysates: HeLa and HEK-293 Cell lysates were detected with anti-Beta Actin (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit (KFA061, blue); anti-Alpha Tubulin (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA062, red); and anti-ANP32B (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA064, green).

    WB of HeLa and HEK-293 cell lysates: HeLa and HEK-293 Cell lysates were detected with anti-Beta Actin (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit (KFA061, blue); anti-Alpha Tubulin (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA062, red); and anti-ANP32B (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA064, green).

FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG2b

Cat No. KFA062

Applications

IF, FC, WB

Reactivity

Mouse

Conjugate

CoraLite® Plus 555

Conjugate:  CoraLite® Plus 555
Size: 

-/ -

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Product Information


Product nameFlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG2b
Assay typeAntibody labeling
Tested applicationsIF, FC, WB
Species ReactivityMouse IgG2b
Antibody amount per labeling reaction0.5 µg antibody
ConjugateCoraLite® Plus 555
Excitation/Emission maxima wavelengths554 nm / 570 nm

Kit Components

Component10 rxns50 rxns200rxns
CoraLite® Plus 555 FlexLinker for Mouse IgG2b
CoraLite® Plus 555 for Mouse IgG2b10ul50ul
FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG2b50rxns
FlexBuffer100ul500ul
FlexQuencher for Mouse IgG2b20ul100ul

Storage Condition

Store for 1 year at -20°C or for 6 months at +4°C upon receipt. Avoid exposure to light

FAQ

Q: What are the FlexLinker, FlexQuencher and FlexBuffer?

A: The FlexLinker is a small polypeptide to which dyes are covalently conjugated that can label unconjugated primary antibodies. The FlexQuencher is an Fc-containing fragment that neutralizes the excess FlexLinker. The FlexBuffer is a PBS-based buffer.


Q: What is the largest quantity I can label?

A: With a standard kit size (50 reactions), you can label 25 µg of one antibody or up to 50 different antibodies. You can easily scale up the antibody amount per labeling approach.


Q: What is the lowest concentration of my primary antibody that I can use?

A: Our protocol uses 0.5 µg of primary antibody in 7 µL, which ends up at 0.07 mg/mL. If the concentration of your antibody is lower, you can also use a larger volume than 7 µL.


Q: Can I label primary antibodies stored in BSA, glycerol, Tris buffer and/or preservatives?

A: Yes, FlexAble Antibody Labeling Kits have been validated with carriers and amine buffers. Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit.


Q: How many different primary antibodies can I label with one kit?

A: You can label up to 50 different antibodies with our FlexAble 50 rxn Kit, and up to 10 antibodies with our FlexAble 10 rxn Kit.


Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?

A: FlexAble labels primary antibodies with a high affinity FlexLinker. Dissociation of FlexLinker from one antibody and association to another antibody is rare. If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies. 


More FAQs


Documentation

SDS
KFA062-SDS FlexAble CoraLite Plus 555 Antibody Labeling Kit
Protocol
Protocol-FlexAble and FlexAble 2.0 Antibody Labeling Kits