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Validation Data Gallery

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  • Western Blot (WB) analysis of MC/9 cells using FceR1a Recombinant monoclonal antibody (84662-4-RR)

    WB analysis of MC/9 using 84662-4-RR

    MC/9 cells were subjected to SDS PAGE followed by western blot with 84662-4-RR (Fc epsilon RI alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of KU812 cells using FceR1a Recombinant monoclonal antibody (84662-4-RR)

    WB analysis of KU812 using 84662-4-RR

    KU812 cells were subjected to SDS PAGE followed by western blot with 84662-4-RR (FceR1a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using FceR1a Recombinant monoclonal antibody (84662-4-RR)

    WB analysis of human testis using 84662-4-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 84662-4-RR (FceR1a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity of 84662-4-RR

    Affinity of 84662-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84662-4-RR against Human FceR1a were performed. The affinity constant is below 1 pM.

FceR1a Recombinant monoclonal antibody

FceR1a Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84662-4-RR
Clone No.241848D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Fc epsilon RI alpha, FCE1A, Fc-epsilon RI-alpha, FcERI, FceRIa

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMC/9 cells, human testis tissue, KU812 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84662-4-RR targets FceR1a in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2022

Product name: Recombinant Human Fc Epsilon RI Alpha protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 26-205 aa of NM_001387280.1

Sequence: VPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQ

相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
Calculated molecular weight30 kDa
Observed molecular weight45-55 kDa
GenBank accession numberNM_001387280.1
Gene Symbol FceR1a
Gene ID (NCBI) 2205
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP12319
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 45- to 55-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation.

Protocols

Product Specific Protocols
WB protocol for FceR1a antibody 84662-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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